désirée cc lines (Shimla Municipal)
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Désirée Cc Lines, supplied by Shimla Municipal, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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1) Product Images from "Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato"
Article Title: Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms242115754
Figure Legend Snippet: Evaluation of earliness for tuberization in Désirée CC lines. ( A ) Image of wild-type (WT) and CC lines of S. tuberosum cv. Désirée growing in the greenhouse. ( B ) Representative images of WT and CC Désirée lines after 12 weeks of transfer to soil. Lower yellow leaves in CC lines indicate their earliness for maturity. The enhanced expression of StCDF1 in these lines (shown later in A) could be the reason. Scale bar = 5 cm. ( C ) In vitro tuber induction experiment (8% sucrose) showed an earliness in transgenic CC Désirée lines compared to WT. ( D ) Average number of stolons per plant after 3-week of transfer to greenhouse. Student’s t -test was used at p < 0.05 (*** p < 0.001, **** p < 0.0001, ns, not significant). Mean values ±SEM are represented in the graph. ( E ) Evaluation of earliness for tuberization in greenhouse after 3-weeks. The graph represents the percentage of plants that formed stolons and/or swollen stolon after three weeks of growth. For panels ( C , E ), no statistical test is performed as the data is plotted as the percentage of nodes or plants tuberizing. For panels ( D , E ), values inside the bars represent the number of plants per line ( n ). #1 and #2 are the two independent lines of respective CC used throughout the studies (as mentioned in ).
Techniques Used: Expressing, In Vitro, Transgenic Assay
Figure Legend Snippet: Expression of tuber marker genes and assessment of tuber productivity in Désirée CC lines under greenhouse conditions. Heat maps depict the relative expression of key tuberization genes in leaves ( A ) and stolons ( B ) of 3-week-old greenhouse plants. Data represents mean of three biological and three technical replicates. Potato EIF3e was used for normalization of gene expression and Ct values are represented next to the respective rows. #1 and #2 are the two independent lines of respective CC used throughout the studies (as mentioned in ). ( C ) Average tuber weight in transgenic Désirée CC lines from 19-week-old plants from greenhouse. The numbers below violin plots represent the number of plants per line (n). In the respective violin plot, a thick central line represents a median and the dotted lines indicate the lower and higher quartiles of the corresponding data points. In panels ( A – C ), Student’s t -test was used at p < 0.05 (* p < 0.05, ** p < 0.001, *** p < 0.005, **** p < 0.0001, ns, not significant). ( D ) Tuber images from greenhouse plants. For imaging, tubers were pooled from 15 plants for each line. Scale bar = 2 cm. The experiment for tuber productivity from greenhouse grown plants was performed two times and similar observations were found. The current data is from the second experiment. Abbreviations: BEL , BEL1-LIKE transcription factor; CDF1, CYCLING DOF FACTOR ; CO2, CONSTANS2 ; GA2OX1, GIBBERELLIN 2 OXIDASE 1 ; IT1, IDENTITY OF TUBER 1 ; PIN4, PIN FORMED 4 ; PTB1/6, POLYPYRIMIDINE TRACT-BINDING PROTEINS 1/6 ; SP5G, SELF-PRUNING 5G ; SP6A, SELF-PRUNING 6A ; SWEET11B, SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTER 11B .
Techniques Used: Expressing, Marker, Gene Expression, Transgenic Assay, Imaging, Binding Assay
Figure Legend Snippet: Evaluation of tuber productivity in Désirée CC lines under field conditions. ( A ) Tuber harvest data of the transgenic Désirée CC plants grown in field conditions for 19 weeks at the ICAR Central Potato Research Institute (CPRI) located at Shimla, India. ( B ) Tuber images of transgenic control and Désirée CC lines. Scale bar = 5 cm. ( C ) Image of transgenic plants growing in the field conditions at CPRI. The field experiment was conducted two times in the year of 2021 and 2022 and similar results were obtained. #1 and #2 are the two independent lines of respective CC used throughout the studies (as mentioned in ). For panel ( A ), Student’s t -test was used at p < 0.05 (* p < 0.05, *** p < 0.001, **** p < 0.0001, ns, not significant). SEM = Standard error of mean. TC = Transgenic control. Figure contains the data obtained from the second field experiment.
Techniques Used: Transgenic Assay, Control
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Expressing:Article Title: Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato Article Snippet: To evaluate the performance of In Vitro:Article Title: Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato Article Snippet: To evaluate the performance of Transgenic Assay:Article Title: Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato Article Snippet: To evaluate the performance of Marker:Article Title: Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato Article Snippet: To evaluate the performance of Gene Expression:Article Title: Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato Article Snippet: To evaluate the performance of Imaging:Article Title: Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato Article Snippet: To evaluate the performance of Binding Assay:Article Title: Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato Article Snippet: To evaluate the performance of Control:Article Title: Tandem Expression of a Mobile RNA and Its RNA-Binding Protein(s) Enhances Tuber Productivity in Potato Article Snippet: To evaluate the performance of |